Dataset Information

Relaxin inhibits patellar tendon healing in rats: a histological and biochemical evaluation.

ABSTRACT:

Background

Female patients are more likely to have tendon injuries than males, especially those who has a higher concentration of relaxin. Previous studies have demonstrated that relaxin attenuates extracellular matrix (ECM) formation. However, the mechanism of relaxin on tendon repair remains unclear. We hypothesize that relaxin inhibits tendon healing by disrupting collagen synthesis.

Methods

A patellar tendon window defect model was established using Sprague-Dawley rats. The center of the patellar tendon was removed from the patella distal apex and inserted to the tibia tuberosity in width of 1 mm. Then, the rats were injected with saline (0.2 μg/kg/day) or relaxin (0.2 μg/kg/day) for two and four weeks, which was followed by biomechanical analysis and histological and histochemical examination.

Results

Mechanical results indicated that relaxin induces a significant decrease in tear resistance, stiffness, and Young's modulus compared to those rats without relaxin treatment. In addition, it was shown that relaxin activates relaxin family peptide receptor 1(RXFP1), disturbs the balance between matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteases (TIMPs), and reduces the deposition of collagen in injury areas.

Conclusions

Relaxin impairs tendon healing in rats. Also, relaxin might lead to tendon injury more commonly for females than males.

SUBMITTER: Xu T

PROVIDER: S-EPMC6661089 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Publications

Relaxin inhibits patellar tendon healing in rats: a histological and biochemical evaluation.

Xu Tianpeng T Bai Jiaxiang J Xu Menglei M Yu Binqing B Lin Jiayi J Guo Xiaobin X Liu Yu Y Zhang Di D Yan Kai K Hu Dan D Hao Yuefeng Y Geng Dechun D

BMC musculoskeletal disorders 20190727 1

<h4>Background</h4>Female patients are more likely to have tendon injuries than males, especially those who has a higher concentration of relaxin. Previous studies have demonstrated that relaxin attenuates extracellular matrix (ECM) formation. However, the mechanism of relaxin on tendon repair remains unclear. We hypothesize that relaxin inhibits tendon healing by disrupting collagen synthesis.<h4>Methods</h4>A patellar tendon window defect model was established using Sprague-Dawley rats. The ce ...[more]

PMID: 31351472

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Project description:ObjectiveTo investigate the efficacy of contrast-enhanced ultrasound (CEUS) in quantitatively evaluating angiogenesis during patellar tendon healing in rats.MethodsA total of 40 Sprague-Dawley rats were used in this study. The patellar tendons of 30 rats (60 limbs) that underwent incision and suture were treated as the operation group and monitored after 7, 14, and 28 days. The normal patellar tendons of 10 rats (20 limbs) were treated as the control group and monitored on day 0. The ultrasound examination was used to evaluate the structure and blood perfusion of the patellar tendon. Immunohistochemistry was used to assess angiogenesis, and the biomechanical test was used to verify functional recovery of the patellar tendon.ResultsThe tendons in the operation group were significantly thickened compared with those in the control group (p < 0.01). The peak intensity (PI) in CEUS of the tendons showed a clear difference at each time point after the surgery (p < 0.01). PI increased in the operation group with a maximum on day 7, and then gradually decreased until day 28 when PI was close to the basic intensity (BI) in the control group (p > 0.05). It was consistent with the change of the CD31-positive staining areas representing angiogenesis of the injured patellar tendons. The PI was positively correlated with the CD31-positive staining area fraction (R = 0.849, p < 0.001). The failure load and tensile strength of the repaired patellar tendons in the operation group increased over time. The PI showed negative correlations with the failure load (R = -0.787, p < 0.001) and tensile strength (R = -0.714, p < 0.001).ConclusionThe PI in CEUS could quantitatively reflect the time-dependent change in the blood supply of the healing site, and the PI correlated with histologic and biomechanical properties of the healing tendon. Quantitative analysis of contrast-enhanced ultrasound could be a useful method to evaluate angiogenesis in healing tendons.

Dataset Information

Relaxin inhibits patellar tendon healing in rats: a histological and biochemical evaluation.

Background

Methods

Results

Conclusions

Publications

Relaxin inhibits patellar tendon healing in rats: a histological and biochemical evaluation.

Similar Datasets

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