Siglec-7 on peripheral blood eosinophils: Surface expression and function.

ABSTRACT:

Background

Siglec-7 is an inhibitory receptor (IR) expressed on human blood eosinophils. Whereas activation of other IRs, including Siglec-8 and CD300a, has been shown to downregulate eosinophil function, little is known about the role of Siglec-7 on human eosinophils.

Objective

To examine Siglec-7 expression and function in eosinophils from normal (ND) and eosinophilic (EO) donors.

Methods

Eosinophil expression of Siglec-7 was quantified by flow cytometry and quantitative PCR. Soluble Siglec-7 (sSiglec-7) levels were measured by ELISA in serum. The effect of Siglec-7 on eosinophil viability and degranulation was assessed in vitro by AnnexinV-FITC/7-AAD staining and by measuring GM-CSF-induced mediator release in culture supernatants. Signal transduction was studied b

SUBMITTER: Legrand F 

PROVIDER: S-EPMC6661208 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature