Flexibility and thermal dynamic stability increase of dsDNA induced by Ru(bpy)₂dppz²⁺ based on AFM and HRM technique.

ABSTRACT: Ru(bpy)₂dppz²⁺ has been widely used as a probe for exploring the structure of double-stranded DNA (dsDNA). The flexibility change of DNA helix is important in many of its biological functions but not well understood. Here, flexibility change of dsDNA helix caused by intercalation with Ru(bpy)₂dppz²⁺ was investigated using the atomic force microscopy. At first, the interactions between ruthenium complex and dsDNA helix were characterized and the binding site size (p = 2.87 bp) and binding constant (K_a = 5.9 * 10⁷ M^-1) were determined by the relative extension of DNA helix using the equation of McGhee and von Hippel. By measuring intercalator-induced DNA elongation and the mean square of end-to-end distance at diffe

SUBMITTER: Jia F 

PROVIDER: S-EPMC6661754 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature