High Proliferative Placenta-Derived Multipotent Cells Express Cytokeratin 7 at Low Level.

ABSTRACT: The purpose of this study was to investigate the immunophenotypes and gene expression profile of high proliferative placenta-derived multipotent cells (PDMCs) population at different stages of culture. We demonstrated that the colonies resulting from single cells were either positive or negative for CK7, whereas only PDMC clones with weak CK7 expression (CK7^low-clones) were highly proliferative. Interestingly, vimentin positive (Vim⁺) placental stromal mesenchymal cells did not express CK7 in situ, but double CK7⁺Vim⁺ cells detection in tissue explants and explants outgrowth indicated CK7 inducible expression in vitro. PCNA presence in CK7⁺Vim⁺ cells during placental explants culturing confirmed belonging of th

SUBMITTER: Shablii V 

PROVIDER: S-EPMC6662495 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature