MetaBAT 2: an adaptive binning algorithm for robust and efficient genome reconstruction from metagenome assemblies.

ABSTRACT: We previously reported on MetaBAT, an automated metagenome binning software tool to reconstruct single genomes from microbial communities for subsequent analyses of uncultivated microbial species. MetaBAT has become one of the most popular binning tools largely due to its computational efficiency and ease of use, especially in binning experiments with a large number of samples and a large assembly. MetaBAT requires users to choose parameters to fine-tune its sensitivity and specificity. If those parameters are not chosen properly, binning accuracy can suffer, especially on assemblies of poor quality. Here, we developed MetaBAT 2 to overcome this problem. MetaBAT 2 uses a new adaptive binning algorithm to eliminate manual parameter tuning. We also performed extensive software engineering op

SUBMITTER: Kang DD 

PROVIDER: S-EPMC6662567 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature