O₂ Reduction to Water by High Potential Multicopper Oxidases: Contributions of the T1 Copper Site Potential and the Local Environment of the Trinuclear Copper Cluster.

ABSTRACT: High potential multicopper oxidases (MCOs) have T1 reduction potentials >600 mV (vs normal hydrogen electrode), making them important catalysts for O₂ reduction in various biotechnological applications. The oxygen reduction mechanism for the low potential MCOs is well-characterized; however, O₂ reactivity of high potential MCOs is not well understood. In this study, we have shown that laccase from Trametes versicolor, where the T1 redox potential is increased by ∼350 mV over that of the low potential MCOs corresponding to an 8 kcal/mol decrease in the driving force, exhibits a slower intramolecular electron transfer (IET) rate to the trinuclear Cu cluster (TNC) in the native intermediate (NI), relative to the low potential MCO from Rhus vernicifera lacca

SUBMITTER: Sekretaryova A 

PROVIDER: S-EPMC6662574 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature