The effects of spatial and temporal replicate sampling on eDNA metabarcoding.

ABSTRACT:

Background

The heterogeneous nature of environmental DNA (eDNA) and its effects on species detection and community composition estimates has been highlighted in several studies in the past decades. Mostly in the context of spatial distribution over large areas, in fewer occasions looking at spatial distribution within a single body of water. Temporal variation of eDNA, similarly, has mostly been studied as seasonality, observing changes over large periods of time, and often only for small groups of organisms such as fish and amphibians.

Methods

We analyzed and compared small-scale spatial and temporal variation by sampling eDNA from two small, isolated dune lakes for 20 consecutive weeks. Metabarcoding was performed on the samples using generic COI primers. Molecular operati

SUBMITTER: Beentjes KK 

PROVIDER: S-EPMC6662575 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature