Circulating microRNA expression profiles associated with systemic lupus erythematosus.

ABSTRACT:

Objective

To evaluate the specificity of expression patterns of cell-free circulating microRNAs (miRNAs) in systemic lupus erythematosus (SLE).

Methods

Total RNA was purified from plasma, and 45 different specific, mature miRNAs were determined using quantitative reverse transcription-polymerase chain reaction assays. A total of 409 plasma samples were obtained from 364 different patients with SLE, healthy control subjects, and control subjects with other autoimmune diseases. The results in the primary cohort of 62 patients with SLE and 29 healthy control subjects were validated in 2 independent cohorts: a validation cohort comprising 68 patients with SLE and 68 healthy control subjects, and a disease control cohort comprising 20 patients with SLE (19 of whom were from the o

SUBMITTER: Carlsen AL 

PROVIDER: S-EPMC6662589 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature