Hybrid nanocomposite as a chest wall graft with improved integration by adipose-derived stem cells.

ABSTRACT: Surgery of the chest wall is potentially required to cover large defects after  removal of malignant tumours. Usually, inert and non-degradable Gore-Tex serves to replace the missing tissue. However, novel biodegradable materials combined with stem cells are available that stimulate the healing. Based on poly-lactic-co-glycolic acid and amorphous calcium phosphate nanoparticles (PLGA/aCaP) and pure PLGA, a dual layer biodegradable hybrid nanocomposite was generated. Mouse adipose-derived stem cells were cultered on electrospun disks (ASCs of C57BL/6), and biomechanical tests were performed. The cell-seeded scaffolds were engrafted in C57BL/LY5.1 mice to serve as a chest wall substitute. Cell invasion into the bi-layered material, extent of CD45⁺ cells, inflammatory response, neo

SUBMITTER: Buschmann J 

PROVIDER: S-EPMC6662805 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature