Particle coating alters mucociliary transit in excised rat trachea: A synchrotron X-ray imaging study.

ABSTRACT: We have previously developed non-invasive in vivo mucociliary transport (MCT) monitoring methods using synchrotron phase contrast X-ray imaging (PCXI) to evaluate potential therapies for cystic fibrosis (CF). However, previous in vivo measurements of MCT velocity using this method were lower than those from alternate methods. We hypothesise this was due to the surface chemistry of the uncoated particles. We investigated the effect of particle surface coating on MCT marker performance by measuring the velocity of uncoated, positively-charged (aminated; NH₂), and negatively-charged (carboxylated; COOH) particles. The effect of aerosolised hypertonic saline (HS) was also investigated, as previous in vivo measurements showed HS significantly increased MCT rate. PCXI experiments were

SUBMITTER: Gardner M 

PROVIDER: S-EPMC6662859 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature