DNA duplex recognition activates Exo1 nuclease activity.

ABSTRACT: Exonuclease 1 (Exo1) is an evolutionarily conserved eukaryotic nuclease that plays a multifaceted role in maintaining genome stability. The biochemical attributes of Exo1 have been extensively characterized via conventional assays. However, the key step governing its activation remains elusive. Extending the previous finding that Exo1 can digest a randomly selected single-stranded DNA (ssDNA) but not a poly(dT) oligonucleotide and using purified recombinant Exo1 and nuclease and electrophoretic mobility shift assays, here we determined that DNA hairpins with a stem size of 4 bp or longer are able to activate Exo1-mediated digestion of ssDNA. We further provide evidence suggesting that Exo1 uses an evolutionarily conserved residue, Lys¹⁸⁵ This residue interacted with the phosphat

SUBMITTER: Li Y 

PROVIDER: S-EPMC6663874 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature