Comparative characterization of human induced pluripotent stem cells (hiPSC) derived from patients with schizophrenia and autism.

ABSTRACT: Human induced pluripotent stem cells (hiPSC) provide an attractive tool to study disease mechanisms of neurodevelopmental disorders such as schizophrenia. A pertinent problem is the development of hiPSC-based assays to discriminate schizophrenia (SZ) from autism spectrum disorder (ASD) models. Healthy control individuals as well as patients with SZ and ASD were examined by a panel of diagnostic tests. Subsequently, skin biopsies were taken for the generation, differentiation, and testing of hiPSC-derived neurons from all individuals. SZ and ASD neurons share a reduced capacity for cortical differentiation as shown by quantitative analysis of the synaptic marker PSD95 and neurite outgrowth. By contrast, pattern analysis of calcium signals turned out to discriminate among healthy control, sc

SUBMITTER: Grunwald LM 

PROVIDER: S-EPMC6663940 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature