All tubulins are not alike: Heterodimer dissociation differs among different biological sources.

ABSTRACT: Tubulin, the subunit of microtubules, is a noncovalent heterodimer composed of one α- and one β-tubulin monomer. Both tubulins are encoded by multiple genes or composed of different isotypes, which are differentially expressed in different tissues and in development. Tubulin αβ dimers are found throughout the eukaryotes and, although very similar, are known to differ among organisms. We seek to investigate tubulins from different tissues and different organisms for a basic physical characteristic: heterodimer stability and monomer exchange between heterodimers. We previously showed that mammalian brain tubulin heterodimers reversibly dissociate, following the mass action law. Dissociation yields native monomers that can exchange with added tubulin to form new heterodimers. Here, we compare

SUBMITTER: Montecinos-Franjola F 

PROVIDER: S-EPMC6664194 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature