Connecting lysosomes and mitochondria - a novel role for lipid metabolism in cancer cell death.

ABSTRACT:

Background

The understanding of lysosomes has been expanded in recent research way beyond their view as cellular trash can. Lysosomes are pivotal in regulating metabolism, endocytosis and autophagy and are implicated in cancer. Recently it was discovered that the lysosomal V-ATPase, which is known to induce apoptosis, interferes with lipid metabolism in cancer, yet the interplay between these organelles is poorly understood.

Methods

LC-MS/MS analysis was performed to investigate lipid distribution in cells. Cell survival and signaling pathways were analyzed by means of cell biological methods (qPCR, Western Blot, flow cytometry, CellTiter-Blue). Mitochondrial structure was analyzed by confocal imaging and electron microscopy, their function was determined by flow cytometry a

SUBMITTER: Bartel K 

PROVIDER: S-EPMC6664539 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature