Reprogramming of Adult Retinal Muller Glial Cells into Human-Induced Pluripotent Stem Cells as an Efficient Source of Retinal Cells.

ABSTRACT: The reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) has broad applications in regenerative medicine. The generation of self-organized retinal structures from these iPSCs offers the opportunity to study retinal development and model-specific retinal disease with patient-specific iPSCs and provides the basis for cell replacement strategies. In this study, we demonstrated that the major type of glial cells of the human retina, Müller cells, can be reprogrammed into iPSCs that acquire classical signature of pluripotent stem cells. These Müller glial cell-derived iPSCs were able to differentiate toward retinal fate and generate concomitantly retinal pigmented epithelial cells and self-forming retinal organoid structures containing retinal progenitor cells. Retinal

SUBMITTER: Slembrouck-Brec A 

PROVIDER: S-EPMC6664555 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature