Cut-C: cleavage under tethered nuclease for conformational capture.

ABSTRACT:

Background

Deciphering the 3D structure of the genome is essential for elucidating the regulatory mechanisms of gene expression in detail. Existing methods, such as chromosome conformation capture (3C) and Hi-C have enabled the identification of novel aspects of chromatin structure. Further identification of protein-centric chromatin conformation is enabled by coupling the Hi-C procedure with a conventional chromatin immunoprecipitation assay. However, these methods are time-consuming and require independent methods for validation.

Results

To simultaneously identify protein-centric chromatin conformation and target protein localization, we have developed Cut-C, a method that combines antibody-mediated cleavage by tethered nuclease with chromosome conformation capture to iden

SUBMITTER: Shimbo T 

PROVIDER: S-EPMC6664727 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature