The role of insulin as a key regulator of seeding, proliferation, and mRNA transcription of human pluripotent stem cells.

ABSTRACT:

Background

Human-induced pluripotent stem cells (hiPSCs) show a great promise as a renewable source of cells with broad biomedical applications. Since insulin has been used in the maintenance of hiPSCs, in this study we explored the role of insulin in culture of these cells.

Methods

We report conditions for insulin starvation and stimulation of hiPSCs. Crystal violet staining was used to study the adhesion and proliferation of hiPSCs. Apoptosis and cell cycle assays were performed through flow cytometry. Protein arrays were used to confirm phosphorylation targets, and mRNA sequencing was used to evaluate the effect of transcriptome.

Results

Insulin improved the seeding and proliferation of hiPSCs. We also observed an altered cell cycle profile and increase in apoptosi

SUBMITTER: Shahbazi M 

PROVIDER: S-EPMC6664730 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature