RpoB, a promising marker for analyzing the diversity of bacterial communities by amplicon sequencing.

ABSTRACT:

Background

Microbiome composition is frequently studied by the amplification and high-throughput sequencing of specific molecular markers (metabarcoding). Various hypervariable regions of the 16S rRNA gene are classically used to estimate bacterial diversity, but other universal bacterial markers with a finer taxonomic resolution could be employed. We compared specificity and sensitivity between a portion of the rpoB gene and the V3 V4 hypervariable region of the 16S rRNA gene.

Results

We first designed universal primers for rpoB suitable for use with Illumina sequencing-based technology and constructed a reference rpoB database of 45,000 sequences. The rpoB and V3 V4 markers were amplified and sequenced from (i) a mock community of 19 bacterial strains from both Gram-negati

SUBMITTER: Ogier JC 

PROVIDER: S-EPMC6664775 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature