Effects of deuteration on transamination and oxidation of hyperpolarized ¹³C-Pyruvate in the isolated heart.

ABSTRACT: This study was designed to determine the effects of deuteration in pyruvate on exchange reactions in alanine aminotransferase (ALT), lactate dehydrogenase (LDH) and flux through pyruvate dehydrogenase (PDH). Although deuteration of a ¹³C enriched substrate is commonly used to increase the lifetime of a probe for hyperpolarization experiments, the potential impact of kinetic isotope effects on such substitutions has not been studied in detail. Metabolism of deuterated pyruvate was investigated in isolated rat hearts. Hearts were perfused with a 1:1 mixture of [U-¹³C₃]pyruvate and [2-¹³C₁]pyruvate or a 1:1 mixture of [U-¹³C₃]pyruvate plus [2-¹³C₁, U-²H₃]pyruvate for 30 min

SUBMITTER: Funk AM 

PROVIDER: S-EPMC6666394 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature