Establishment of human trabecular meshwork cell cultures using nontransplantable corneoscleral rims.

ABSTRACT: Human trabecular meshwork (hTM) cell isolation in academic settings utilizes the motile nature of these cells, allowing them to migrate away from the explant and proliferate on distal regions of the culture substrate. Corneoscleral rims used for transplantation are a potential source of explants for the establishment of hTM cell cultures. However, cell isolation and the initiation of primary cell cultures from ocular tissues stored in Optisol-GS medium for an extended period of time (>6 days) has proven difficult, since Optisol-GS remarkably reduces cell viability and cellularity. Therefore, explants obtained from ocular tissues stored in Optisol-GS do not often provide adequate cell yield to initiate primary cell cultures if conventional culture techniques are used. Therefore, the majorit

SUBMITTER: Waduthanthri KD 

PROVIDER: S-EPMC6667097 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature