Global transcriptomic analysis of the arcuate nucleus following chronic glucocorticoid treatment.

ABSTRACT:

Objective

Glucocorticoids (GCs) are widely prescribed medications that are well recognized to cause adverse metabolic effects including hyperphagia, obesity, and hyperglycemia. These effects have been recapitulated in a murine model of GC excess, and we hypothesize that they are mediated, in part, through central mechanisms. This study aimed to identify genes in the hypothalamic arcuate nucleus (ARC) that are altered with GC treatment and evaluate their contribution to GC-induced metabolic abnormalities.

Methods

Corticosterone (Cort; 75 μg/ml) was administered in the drinking water to male C57Bl/6J mice for 2 days or 4 weeks. Phenotypic analysis of each group was undertaken and central and peripheral tissues were collected for biochemical and mRNA analyses. Arcuate nuclei we

SUBMITTER: Wray JR 

PROVIDER: S-EPMC6667392 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature