A high-speed search engine pLink 2 with systematic evaluation for proteome-scale identification of cross-linked peptides.

ABSTRACT: We describe pLink 2, a search engine with higher speed and reliability for proteome-scale identification of cross-linked peptides. With a two-stage open search strategy facilitated by fragment indexing, pLink 2 is ~40 times faster than pLink 1 and 3~10 times faster than Kojak. Furthermore, using simulated datasets, synthetic datasets, ¹⁵N metabolically labeled datasets, and entrapment databases, four analysis methods were designed to evaluate the credibility of ten state-of-the-art search engines. This systematic evaluation shows that pLink 2 outperforms these methods in precision and sensitivity, especially at proteome scales. Lastly, re-analysis of four published proteome-scale cross-linking datasets with pLink 2 required only a fraction of the time used by pLink 1, with up to

SUBMITTER: Chen ZL 

PROVIDER: S-EPMC6667459 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature