Multi-omics insights into functional alterations of the liver in insulin-deficient diabetes mellitus.

ABSTRACT:

Objective

The liver regulates the availability of insulin to other tissues and is the first line insulin response organ physiologically exposed to higher insulin concentrations than the periphery. Basal insulin during fasting inhibits hepatic gluconeogenesis and glycogenolysis, whereas postprandial insulin peaks stimulate glycogen synthesis. The molecular consequences of chronic insulin deficiency for the liver have not been studied systematically.

Methods

We analyzed liver samples of a genetically diabetic pig model (MIDY) and of wild-type (WT) littermate controls by RNA sequencing, proteomics, and targeted metabolomics/lipidomics.

Results

Cross-omics analyses revealed increased activities in amino acid metabolism, oxidation of fatty acids, ketogenesis, and gluconeog

SUBMITTER: Backman M 

PROVIDER: S-EPMC6667734 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature