Plucked hair follicles from patients with chronic discoid lupus erythematosus show a disease-specific molecular signature.

ABSTRACT:

Objective

When faced with clinical symptoms of scarring alopecia-the standard diagnostic pathway involves a scalp biopsy which is an invasive and expensive procedure. This project aimed to assess if plucked hair follicles (HFs) containing living epithelial cells can offer a non-invasive approach to diagnosing inflammatory scalp lesions.

Methods

Lesional and non-lesional HFs were extracted from the scalp of patients with chronic discoid lupus erythematosus (CDLE), psoriasis and healthy controls. RNA was isolated from plucked anagen HFs and microarray, as well as quantitative real-time PCR was performed.

Results

Here, we report that gene expression analysis of only a small number of HF plucked from lesional areas of the scalp is sufficient to differentiate CDLE from pso

SUBMITTER: Shalbaf M 

PROVIDER: S-EPMC6667780 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature