Continuous integrated antibody precipitation with two-stage tangential flow microfiltration enables constant mass flow.

ABSTRACT: Continuous precipitation is a new unit operation for the continuous capture of antibodies. The capture step is based on continuous precipitation with PEG6000 and Zn⁺⁺ in a tubular reactor integrated with a two-stage continuous tangential flow filtration unit. The precipitate cannot be separated with centrifugation, because a highly compressed sediment results in poor resolubilization. We developed a new two-stage tangential flow microfiltration method, where part of the concentrated retentate of the first stage was directly fed to the second stage, together with the wash buffer. Thus, the precipitate was concentrated and washed in a continuous process. We obtained 97% antibody purity, a 95% process yield during continuous operation, and a fivefold reduction in pre-existing high-

SUBMITTER: Burgstaller D 

PROVIDER: S-EPMC6667901 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature