Differential contributions of NO₃ ^-/NH₄ ⁺ to nitrogen use in response to a variable inorganic nitrogen supply in plantlets of two Brassicaceae species in vitro.

ABSTRACT:

Background

The primary sources of nitrogen for plants have been suggested to be nitrate (NO₃ ^-) and ammonium (NH₄ ⁺). However, when both nitrate and ammonium are simultaneously available to plants, it is very difficult to differentially quantify NO₃ ^-/NH₄ ⁺ utilization in culture media or soil. Consequently, the contribution of NO₃ ^-/NH₄ ⁺ to total inorganic nitrogen assimilation cannot be determined.

Results

We developed a method called the bidirectional stable nitrogen isotope tracer to differentially quantify the nitrate and ammonium utilization by Orychophragmus violaceus (Ov) and Brassica napus (Bn) plantlets in

SUBMITTER: Zhang K 

PROVIDER: S-EPMC6668107 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature