RNA-seq-based selection of reference genes for RT-qPCR analysis of pitaya.

ABSTRACT: Reverse-transcription quantitative real-time PCR (RT-qPCR) is a primary tool for measuring gene expression levels, and selection of appropriate reference genes is crucial for accurate and reproducible results of gene expression under various experimental conditions. However, no systematic evaluation of reference genes in pitaya (Hylocereus undatus Britt.) has been performed. Here, we examined the expression of five candidate reference genes, namely elongation factor 1-alpha (HuEF1-α), 18S ribosomal RNA (Hu18S rRNA), ubiquitin (HuUBQ), actin (HuACT), and ubiquitin-conjugating enzyme (HuUQT), under different conditions in pitaya. The expression stabilities of these five genes were evaluated using two computation programs: geNorm and NormFinder. The results were further validated by normalizi

SUBMITTER: Nong Q 

PROVIDER: S-EPMC6668369 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature