A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos.

ABSTRACT: RNA granules are subcellular compartments that are proposed to form by liquid-liquid phase separation (LLPS), a thermodynamic process that partitions molecules between dilute liquid phases and condensed liquid phases. The mechanisms that localize liquid phases in cells, however, are not fully understood. P granules are RNA granules that form in the posterior of Caenorhabditis elegans embryos. Theoretical studies have suggested that spontaneous LLPS of the RNA-binding protein PGL-3 with RNA drives the assembly of P granules. We find that the PGL-3 phase is intrinsically labile and requires a second phase for stabilization in embryos. The second phase is formed by gel-like assemblies of the disordered protein MEG-3 that associate with liquid PGL-3 droplets in the embryo posterior. Co-assembl

SUBMITTER: Putnam A 

PROVIDER: S-EPMC6668929 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature