TPC2-mediated Ca²⁺ signaling is required for the establishment of synchronized activity in developing zebrafish primary motor neurons.

ABSTRACT: During the development of the early spinal circuitry in zebrafish, spontaneous Ca²⁺ transients in the primary motor neurons (PMNs) are reported to transform from being slow and uncorrelated, to being rapid, synchronized and patterned. In this study, we demonstrated that in intact zebrafish, Ca²⁺ release via two-pore channel type 2 (TPC2) from acidic stores/endolysosomes is required for the establishment of synchronized activity in the PMNs. Using the SAIGFF213A;UAS:GCaMP7a double-transgenic zebrafish line, Ca²⁺ transients were visualized in the caudal PMNs (CaPs). TPC2 inhibition via molecular, genetic or pharmacological means attenuated the CaP Ca²⁺ transients, and decreased the normal ipsilateral correlation and contralateral anti-correlation,

SUBMITTER: Kelu JJ 

PROVIDER: S-EPMC6669046 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature