Ubiquitous Carbohydrate Binding Modules Decorate 936 Lactococcal Siphophage Virions.

ABSTRACT: With the availability of an increasing number of 3D structures of bacteriophage components, combined with powerful in silico predictive tools, it has become possible to decipher the structural assembly and functionality of phage adhesion devices. In the current study, we examined 113 members of the 936 group of lactococcal siphophages, and identified a number of Carbohydrate Binding Modules (CBMs) in the neck passage structure and major tail protein, on top of evolved Dit proteins, as recently reported by us. The binding ability of such CBM-containing proteins was assessed through the construction of green fluorescent protein fusion proteins and subsequent binding assays. Two CBMs, one from the phage tail and another from the neck, demonstrated definite binding to their phage-specific host

SUBMITTER: Hayes S 

PROVIDER: S-EPMC6669499 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature