Label-free quantitative proteomics in Candida yeast species: technical and biological replicates to assess data reproducibility.

ABSTRACT:

Objective

Label-free quantitative proteomics has emerged as a powerful strategy to obtain high quality quantitative measures of the proteome with only a very small quantity of total protein extract. Because our research projects were requiring the application of bottom-up shotgun mass spectrometry proteomics in the pathogenic yeasts Candida glabrata and Candida albicans, we performed preliminary experiments to (i) obtain a precise list of all the proteins for which measures of abundance could be obtained and (ii) assess the reproducibility of the results arising respectively from biological and technical replicates.

Data description

Three time-courses were performed in each Candida species, and an alkaline pH stress was induced for two of them. Cells were collected 10 and 60

SUBMITTER: Lelandais G 

PROVIDER: S-EPMC6669970 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature