Inhibition of phosphoenolpyruvate carboxykinase blocks lactate utilization and impairs tumor growth in colorectal cancer.

ABSTRACT:

Background

Metabolic reprogramming is a key feature of malignant cells. While glucose is one of the primary substrates for malignant cells, cancer cells also display a remarkable metabolic flexibility. Depending on nutrient availability and requirements, cancer cells will utilize alternative fuel sources to maintain the TCA cycle for bioenergetic and biosynthetic requirements. Lactate was typically viewed as a passive byproduct of cancer cells. However, studies now show that lactate is an important substrate for the TCA cycle in breast, lung, and pancreatic cancer.

Methods

Metabolic analysis of colorectal cancer (CRC) cells was performed using a combination of bioenergetic analysis and ¹³C stable isotope tracing.

Results

We show here that CRC cells use lact

SUBMITTER: Montal ED 

PROVIDER: S-EPMC6670241 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature