Improved quantitation of lipid classes using supercritical fluid chromatography with a charged aerosol detector.

ABSTRACT: Quantitatively and rapidly analyzing lipids is necessary to elucidate their biological functions. Herein, we developed a quantitative method for various lipid classes using supercritical fluid chromatography (SFC) coupled with a charged aerosol detector (CAD), providing high-throughput data analysis to detect a large number of molecules in each lipid class as one peak. Applying the CAD was useful for analyzing lipid molecules in the same lipid class with a constant response under the same mobile phase composition. First, we optimized the washing method for the diethylamine column, achieving baseline separation of lipid classes while maintaining good peak shapes. In addition, the CAD conditions (organic solvent evaporation and numerical correction of the CAD data) were optimized to improve

SUBMITTER: Takeda H 

PROVIDER: S-EPMC6672046 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature