Two Distinct E2F Transcriptional Modules Drive Cell Cycles and Differentiation.

ABSTRACT: Orchestrating cell-cycle-dependent mRNA oscillations is critical to cell proliferation in multicellular organisms. Even though our understanding of cell-cycle-regulated transcription has improved significantly over the last three decades, the mechanisms remain untested in vivo. Unbiased transcriptomic profiling of G₀, G₁-S, and S-G₂-M sorted cells from FUCCI mouse embryos suggested a central role for E2Fs in the control of cell-cycle-dependent gene expression. The analysis of gene expression and E2F-tagged knockin mice with tissue imaging and deep-learning tools suggested that post-transcriptional mechanisms universally coordinate the nuclear accumulation of E2F activators (E2F3A) and canonical (E2F4) and atypical (E2F8) repressors during the cell cycle in 

SUBMITTER: Cuitino MC 

PROVIDER: S-EPMC6673649 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature