Effects of Transient Exposure to High Shear on Neutrophil Rolling Behavior.

ABSTRACT:

Introduction

Neutrophils display an array of behaviors ranging from rolling and migration to phagocytosis and granule secretion. Several of these behaviors are modulated by the local shear conditions. In the normal circulation, neutrophils experience shear rates from approximately 10-2,000 s^-1. However, neutrophils are also exposed to pathological shear levels in natural conditions such as severe stenosis and arteriosclerosis, as well as in blood-contacting devices such as ventricular assist devices (VADs) and hemodialysis machines. The effects of transiently (< 1 sec) exposing neutrophils to abnormally high shear rates (>3,000 s^-1) are not well understood.

Methods

We developed a set of microfluidic devices capable of exposing neutrophils to high shear

SUBMITTER: Lewis CS 

PROVIDER: S-EPMC6673669 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature