Per-sample immunoglobulin germline inference from B cell receptor deep sequencing data.

ABSTRACT: The collection of immunoglobulin genes in an individual's germline, which gives rise to B cell receptors via recombination, is known to vary significantly across individuals. In humans, for example, each individual has only a fraction of the several hundred known V alleles. Furthermore, the currently-accepted set of known V alleles is both incomplete (particularly for non-European samples), and contains a significant number of spurious alleles. The resulting uncertainty as to which immunoglobulin alleles are present in any given sample results in inaccurate B cell receptor sequence annotations, and in particular inaccurate inferred naive ancestors. In this paper we first show that the currently widespread practice of aligning each sequence to its closest match in the full set of IMGT allel

SUBMITTER: Ralph DK 

PROVIDER: S-EPMC6675132 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature