Transcriptomic Profiling of the Developing Cardiac Conduction System at Single-Cell Resolution.

ABSTRACT:

Rationale

The cardiac conduction system (CCS) consists of distinct components including the sinoatrial node, atrioventricular node, His bundle, bundle branches, and Purkinje fibers. Despite an essential role for the CCS in heart development and function, the CCS has remained challenging to interrogate because of inherent obstacles including small cell numbers, large cell-type heterogeneity, complex anatomy, and difficulty in isolation. Single-cell RNA-sequencing allows for genome-wide analysis of gene expression at single-cell resolution.

Objective

Assess the transcriptional landscape of the entire CCS at single-cell resolution by single-cell RNA-sequencing within the developing mouse heart.

Methods and results

Wild-type, embryonic day 16.5 mouse hearts (n=6 per zone)

SUBMITTER: Goodyer WR 

PROVIDER: S-EPMC6675655 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature