Study of the mechanism by which dinaciclib induces apoptosis and cell cycle arrest of lymphoma Raji cells through a CDK1-involved pathway.

ABSTRACT:

Objective

This study aimed to identify and evaluate the mechanism by which apoptosis and cell cycle arrest were induced by dinaciclib in lymphoma Raji cells.

Methods

The colony formation assay was used to detect cell proliferation of Raji cells. Cell cycle arrest and cell apoptosis were determined by flow cytometry and TUNEL assays, respectively. Protein expression related to the Raji cell state was evaluated by Western blot. The Raji/Dinaciclib drug-resistant cell line was established, where the regulating functions of CDK1-involved pathway were verified. In addition, the effect of dinaciclib in vivo was examined in orthotopically implanted tumors in nude mice.

Results

Cell apoptosis was induced, and DNA synthesis ability was decreased in a time-dependent manner in d

SUBMITTER: Zhao H 

PROVIDER: S-EPMC6675732 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature