Reparative macrophage transplantation for myocardial repair: a refinement of bone marrow mononuclear cell-based therapy.

ABSTRACT: Reparative macrophages play an important role in cardiac repair post-myocardial infarction (MI). Bone marrow mononuclear cells (BM-MNCs) have been investigated as a donor for cell therapy but with limited clinical success. These cells, however, may be utilized as a source for reparative macrophages. This translational study aimed to establish a robust in vitro protocol to produce functional reparative macrophages from BM-MNCs and to establish pre-clinical evidence of the efficacy of reparative macrophage transplantation for the treatment of MI. Mouse BM-MNCs were treated with M-CSF plus IL-4, IL-10, TGF-β1 or combinations of these in vitro. The concomitant administration of M-CSF and IL-4 produced the highest rate and largest number of CD11b⁺F4/80⁺CD206⁺ re

SUBMITTER: Podaru MN 

PROVIDER: S-EPMC6675756 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature