Temporal Proteomic Analysis of BK Polyomavirus Infection Reveals Virus-Induced G₂ Arrest and Highly Effective Evasion of Innate Immune Sensing.

ABSTRACT: BK polyomavirus (BKPyV) is a small DNA virus that establishes a life-long persistent infection in the urinary tract of most people. BKPyV is known to cause severe morbidity in renal transplant recipients and can lead to graft rejection. The simple 5.2-kbp double-stranded DNA (dsDNA) genome expresses just seven known proteins; thus, it relies heavily on the host machinery to replicate. How the host proteome changes over the course of infection is key to understanding this host-virus interplay. Here, for the first time quantitative temporal viromics has been used to quantify global changes in >9,000 host proteins in two types of primary human epithelial cells throughout 72 h of BKPyV infection. These data demonstrate the importance of cell cycle progression and pseudo-G₂ arrest in

SUBMITTER: Caller LG 

PROVIDER: S-EPMC6675895 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature