Gut microbiota promote the inflammatory response in the pathogenesis of systemic lupus erythematosus.

ABSTRACT:

Objectives

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease whose onset and progression are affected by genetic and environmental factors. The purpose of this study is to identify the influence of gut microbiota in the pathogenesis of SLE, and to investigate the mechanism involved.

Methods

Fecal microbiota from C57/BL6 mice and SLE prone mice were examined using next-generation sequencing (NGS). Germ free mice were given fecal microbiota transplantation (FMT), and their gut microbiome and gene expression in recipients' colons were examined by NGS. The anti-double stranded DNA (anti-dsDNA) antibodies in recipients were determined using an enzyme-linked immunosorbent assay (ELISA). The immune cell profiles of mice were analyzed by flow cytometry at the 3rd we

SUBMITTER: Ma Y 

PROVIDER: S-EPMC6676588 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature