Diversity and immune responses against Plasmodium falciparum gametocytes in non-febrile school children living in Southern Ghana.

ABSTRACT:

Background

Natural exposure to gametocytes can result in the development of immunity against the gametocyte by the host as well as genetic diversity in the gametocyte. This study evaluated the quantity and quality of natural immune responses against a gametocyte antigen, Pfs230 as well as the prevalence and diversity of gametocytes circulating in children living in two communities in southern Ghana.

Methods

Whole blood (2.5 ml) was collected from 137 non-febrile school children aged between 6 and 12 years old quarterly for a 6-month period. A drop of blood was used to prepare thick and thin blood films for parasite prevalence and density estimation. Subsequently, stored plasma samples were used in ELISAs assays to measure antibody responses and avidity against Pfs230. RNA wa

SUBMITTER: Amoah LE 

PROVIDER: S-EPMC6676606 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature