A rapid preparation procedure for laser microdissection-mediated harvest of plant tissues for gene expression analysis.

ABSTRACT:

Background

Gene expression changes that govern essential biological processes can occur at the cell-specific level. To gain insight into such events, laser microdissection is applied to cut out specific cells or tissues from which RNA for gene expression analysis is isolated. However, the preparation of plant tissue sections for laser microdissection and subsequent RNA isolation usually involves fixation and embedding, processes that are often time-consuming and can lower the yield and quality of isolated RNA.

Results

Infection sites of the parasitic plant Cuscuta reflexa growing on its compatible host plant Pelargonium zonale were sectioned using a vibratome and dried on glass slides at 4 °C before laser microdissection. High quality RNA (RQI > 7) was isolated

SUBMITTER: Olsen S 

PROVIDER: S-EPMC6676614 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature