Intracellular MicroRNA imaging using telomerase-catalyzed FRET ratioflares with signal amplification.

ABSTRACT: Intracellular microRNA (miRNA) detection has attracted increasing attention, resulting in significant achievements. However, the development of an available tool that possesses a satisfactory signal-to-background ratio and high sensitivity for miRNA detection remains challenging. Herein, a class of telomerase-catalyzed FRET (fluorescence resonance energy transfer) ratioflares has been developed for the accurate sensing of low-abundance cancer-related miRNA both in a fluorescence assay and living cell imaging with signal amplification capacity. In this work, endogenous telomerase is led in a miRNA test system with signal amplification for the first time, wherein telomerase extends hexamer telomeric repeats (TTAGGG) using the 3' end of the capture probe as the primer. The synergetic work of

SUBMITTER: Xian L 

PROVIDER: S-EPMC6677110 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature