PUMILIO, but not RBMX, binding is required for regulation of genomic stability by noncoding RNA NORAD.

ABSTRACT: NORAD is a conserved long noncoding RNA (lncRNA) that is required for genome stability in mammals. NORAD acts as a negative regulator of PUMILIO (PUM) proteins in the cytoplasm, and we previously showed that loss of NORAD or PUM hyperactivity results in genome instability and premature aging in mice (Kopp et al., 2019). Recently, however, it was reported that NORAD regulates genome stability through an interaction with the RNA binding protein RBMX in the nucleus. Here, we addressed the contributions of NORAD:PUM and NORAD:RBMX interactions to genome maintenance by this lncRNA in human cells. Extensive RNA FISH and fractionation experiments established that NORAD localizes predominantly to the cytoplasm with or without DNA damage. Moreover, g

SUBMITTER: Elguindy MM 

PROVIDER: S-EPMC6677556 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature