Transcriptomics reveals complex kinetics of dorsal-ventral patterning gene expression in the mandibular arch.

ABSTRACT: The mandibular or first pharyngeal arch forms the upper and lower jaws in all gnathostomes. A gene regulatory network that defines ventral, intermediate, and dorsal domains along the dorsal-ventral (D-V) axis of the arch has emerged from studies in zebrafish and mice, but the temporal dynamics of this process remain unclear. To define cell fate trajectories in the arches we have performed quantitative gene expression analyses of D-V patterning genes in pharyngeal arch primordia in zebrafish and mice. Using NanoString technology to measure transcript numbers per cell directly we show that, in many cases, genes expressed in similar D-V domains and induced by similar signals vary dramatically in their temporal profiles. This suggests that cellular responses to D-V patterning signals are likel

SUBMITTER: Sharma PP 

PROVIDER: S-EPMC6677562 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature