SPECTRUM - A MATLAB Toolbox for Proteoform Identification from Top-Down Proteomics Data.

ABSTRACT: Top-Down Proteomics (TDP) is an emerging proteomics protocol that involves identification, characterization, and quantitation of intact proteins using high-resolution mass spectrometry. TDP has an edge over other proteomics protocols in that it allows for: (i) accurate measurement of intact protein mass, (ii) high sequence coverage, and (iii) enhanced identification of post-translational modifications (PTMs). However, the complexity of TDP spectra poses a significant impediment to protein search and PTM characterization. Furthermore, limited software support is currently available in the form of search algorithms and pipelines. To address this need, we propose 'SPECTRUM', an open-architecture and open-source toolbox for TDP data analysis. Its salient features include: (i) MS2-based intact

SUBMITTER: Basharat AR 

PROVIDER: S-EPMC6677810 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature