Methanogenic Degradation of Long n-Alkanes Requires Fumarate-Dependent Activation.

ABSTRACT: Methanogenic degradation of n-alkanes is prevalent in n-alkane-impacted anoxic oil reservoirs and oil-polluted sites. However, little is known about the initial activation mechanism of the substrate, especially n-alkanes with a chain length above C₁₆ Here, a methanogenic C₁₆ to C₂₀n-alkane-degrading enrichment culture was established from production water of a low-temperature oil reservoir. At the end of the incubation (364 days), C₁₆ to C₂₀ (1-methylalkyl)succinates were detected in the n-alkane-amended enrichment culture, suggesting that fumarate addition had occurred in the degradation process. This evidence is supported further by the positive amplification of the assA gene encoding the alp

SUBMITTER: Ji JH 

PROVIDER: S-EPMC6677850 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature