Identification and Characterization of MiRNAs in Coccomyxa subellipsoidea C-169.

ABSTRACT: Coccomyxa subellipsoidea C-169 (C-169) is an oleaginous microalga which is promising for renewable biofuel production. MicroRNAs (miRNAs), as the pivotal modulators of gene expression at post-transcriptional level, are prospective candidates for bioengineering practice. However, so far, no miRNA in C-169 has been reported and its potential impact upon CO₂ supplementation remains unclear. High-throughput sequencing of small RNAs from C-169 cultured in air or 2% CO₂ revealed 124 miRNAs in total, including 118 conserved miRNAs and six novel ones. In total, 384 genes were predicted as their potential target genes, 320 for conserved miRNAs and 64 for novel miRNAs. The annotated target genes were significantly enriched in six KEGG pathways, including pantothenate and

SUBMITTER: Yang R 

PROVIDER: S-EPMC6678167 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature