Morphological State Transition Dynamics in EGF-Induced Epithelial to Mesenchymal Transition.

ABSTRACT: Epithelial to Mesenchymal Transition (EMT) is a multi-state process. Here, we investigated phenotypic state transition dynamics of Epidermal Growth Factor (EGF)-induced EMT in a breast cancer cell line MDA-MB-468. We have defined phenotypic states of these cells in terms of their morphologies and have shown that these cells have three distinct morphological states-cobble, spindle, and circular. The spindle and circular states are the migratory phenotypes. Using quantitative image analysis and mathematical modeling, we have deciphered state transition trajectories in different experimental conditions. This analysis shows that the phenotypic state transition during EGF-induced EMT in these cells is reversible, and depends upon the dose of EGF and level of phosphorylation of the EGF receptor

SUBMITTER: Devaraj V 

PROVIDER: S-EPMC6678216 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature